Instead of looking at the PC, users can keep their eyes on the microscope, adjusting the control dial with one hand. Pulse-free flow is critical for generating high quality and repeatable results. Mixed pressure ranges can be connected to the same board (positive and negative pressures, and Push-Pull). Based on the patented FASTABTM technology, our pressure and flow controllers ensure a stable and pulseless flow to enhance accuracy and reproducibility in the experiments. Fluigent has developed a pressure-based technology that offers an integrated pressure supply and control (positive and negative pressure) in a light (< 170 g) and compact format (L*l*H = 7*5*4). 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, LineUp Series, the new generation of microfluidic controllers, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, Dry, oil-free gas, air, any non corrosive or non explosive gas, Aqueous solvent, oil, organic solent, biological sample, Control in real-time, protocol automation, data record and export. Instead of looking at the PC, users can keep their eyes on the microscope, adjusting the control dial with one hand. It can be used without a PC or controlled with Fluigent Software Solutions to benefit from control in real-time, protocol automation, graphic displays and custom integration. Excellent response time, pulseless and highly stable, Configurable with pressure and switch control modules, Standalone platform adapted to industrial uses. When combined with theLINKmodule, thePush-Pullcapabilities are extended by using Fluigent software to control the system, or to generate time based protocols and reccord data(OxyGEN software). This allows the user to set a flow rate directly on the instrument display. In addition, a microfluidic pressure pump can easily be integrated into a setup with the Software Development Kit (SDK). The addition of aflow sensorenables one tocontrol or monitor flow rateas well asdispense volume. Control without a PC using the Push-Pull hardware interface with one hand. 
Fluigent was the first company to introduce pressure-based flow control to handle fluids in microfluidics. Liquid flow rate ranges from a few nL/min to 5 mL/min.
Range available from -800 mbar for vacuum aspiration and up to 7 bar for pressure. Fluigent is an international company which develops, manufactures and supports the most advanced fluid control systems available for microfluidics offering greater control, automation, precision, and ease of use.. Improved reliability and reproducibility of results are possible due to the pulseless flow. Our innovative pressure-based microfluidic controllers are compatible with lab on a chip device and a wide variety of microfluidic technologies that will allow you to focus on the science, not on the setup. Control without a PC using the Flow EZ hardware interface with one hand. Improved reliability and reproducibility of of results is possible due to the pulseless flow. The F-OEM makes use of Fluigents patented Fastab 2 technology. We offer best-in-class standard & custom OEM components, system development, and engineering services, with strong expertise in fluid management to accelerate your development process. The product line can detect values over the range of-1000 mbar(-15 psi) to7000 mbar(100 psi). The entire system can be controlled without a PC using local control and can also be monitored by Fluigent Software to extend its capabilities and benefit from automation. Select modules you need and combine them together. Fig 2: (left) Pneumatic schematic of a standard fluidic system using pressure to move fluids (right) Pneumatic schematic of Fluigent new technology. With the addition of a pressure leakage component, the pressure drop now takes about 5 seconds (see Fig. The field-proven technology ensures to obtain quickly reliable and superior results for your experiments. Combine as many modules as you want to fit your application needs. Combine up to 12 modules as your work flow grows. ThePush-Pullmodules, are available in different pressure and vacuum ranges, to provide the optimum level of pressure control and resolution. The applied pressure will automatically adjust to maintain the flow rate. The use of pressure to handle fluids also provides a quick response time allowing for precise operations such as stop flow and accurate pressure/flow rate steps. For point-of-care applications, a touch screen can be developed based on your needs. How to set the Flow EZ (power and pressure supply), Fluidic and pneumatic connection to the microfluidic setup, Extend the pressure system by adding modules, How to send and apply an order to pressurize a reservoir, How to disconnect a module to modify the pressure system, Add a flow sensor to control directly in flow rate, Switch the module OFF and stop the experiment, 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, A human BBB (blood brain barrier)-on-chip to assess vascular permeability, Doing PhD with Fluigent 2021: Research on Honey by Daniel Kraus (Jena, Germany), Hans-Knll-Institut, New Antibiotics, Cultivation in Droplets, Microfluidic white paper An exploration of Microfluidics and fluid handling, LineUp Series, the new generation of microfluidic controllers, The Hebrew University: Single-cell encapsulation and culture in 3D hydrogels followed by InDrops / Drop-Seq microfluidic protocols for RNA profiling, University of Cambridge: Giant unilamellar vesicle production and testing, The Micro/Nano Bioelectronics and Biosensors (MBIOS) from Tianjin University, Peristaltic pump vs pressure-based microfluidic flow control systems for Organ on-chip applications, E. Coli culture in droplets using dSURF fluorosurfactant, Characterization of copper electrodeposition in liquid phase electron microscopy, Production of water-in-oil emulsions using a droplet generator chip, Droplet generation using syringe pumps and pressure-based flow controllers, Comparison between peristaltic, syringe and pressure pumps for microfluidic applications, University of Maryland: A soft robotic hand with integrated fluidic circuitry that can play Nintendo, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, 0,1% on the measured value (effective beyond 10% of the maximum pressure), Dry, oil-free gas, air, any non corrosive or non explosive gas, Aqueous solvent, oil, organic solent, biological sample, Control in real-time, protocol automation, data record and export. The microfluidic laboratories and industry were struggling to perform their research and develop equipment to the level and precision required in terms of fluid control. Configure your own system: The F-OEM consists of a main board to which one can add pressure and switch control modules depending on the configuration required. The addition of aFLOW UNITenables one tocontrol or monitor flow rateas well asmeasure a dispensed volume. The technology is powered by an embedded rechargeable battery to facilitate portability. The MFCS can be controlled by Fluigent software to automate your protocols. The principle of pressure actuation in microfluidic systems is shown in the figure. Fluigent pressure and flow controllers bring this performance and benefits to the system. The MFCS system can provide negative or positive pressures as needed by using up to four or eight independently controllable channels. A photonic technology institute tests a variety of lab-on-chip systems, the number of flow channels and the required pressures vary from one test to another. Benefit from the best performance and Fluigent expertise for your project. We offer modern microfluidic systems and components that enhance productivity. The delivered software package is suitable for Windows and Linux platforms. Our engineered systems tackle these limitations by bringing a cost-effective solution with a highly reduced footprint. Custom software application: Public SDK libraries are available on GitHub for all integration into the users proprietary software, Adapted to cell culture or other experiments involving resistance variations: the algorithm adjusts its model to the setup resistance in real-time, Save precious sample or reagent: reduced time to reach desired flow rates uses less liquid during a calibration step, Save time with reduced settling time and no calibration. Support reservoir sizes from 2 mL to one liter laboratory bottles. Fluigent developed the patented FASTAB technology: a pressure-driven technology that has no moving parts and includes an advanced feedback control algorithm to maintain precise pressure control. Pulse-free flow is critical for generating high quality and repeatable results. Two pressure modules consisting of a 0 2000 mbar and -800 + 1000 mbar are connected to the main F-OEM board for pressurizing two fluidic reservoirs, allowing for pressure-based fluid handling. There are many advantages to direct control: Configuration 1: 2 pressure modules. One can be operational and generate data rapidly. Two pressure modules consisting of a 0 2000 mbar and -800 + 1000 mbar are connected to the main F-OEM board for pressurizing two fluidic reservoirs, allowing for pressure-based fluid handling. For more information or a technical discussion, 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, Explore our 15 years of field-proven experience, Developing a new fluidic system? The FASTAB microfluidic patented technology used by MFCS series avoids cross contamination as there is no direct contact between the instrument and the reagents. It is at the heart of the Fluigents next generation performance providing the fastest, most stable and compact system for microfluidics available.
The pressure automatically adjusts in the background to maintain the flow rate. TheLineUpPush-Pullis a standalone controller with the ability to deliverfinely regulated pressureor avacuumthrough a single outlet over the range of-800 to +1000 mbar. The Fluigent LineUp series, including the new push-pull pump, enables precise and highly controlled aspiration and respiration of liquids. Each channel can be controlled independently and deliver a specific amount of pressure or vacuum to handle fluids. Each P-SWITCH module requires 2 supplied sources overthe range or -800 mbar to 2 bar. This technology can be integrated in any custom project. Its improved reactivity allows countering, in real-time, the interactions between microfluidic channels in complex situations. Conventional water monitoring is based on laboratory instruments that are generally sophisticated and expensive. Integrated flow control: To switch from pressure control to flow rate control, one can directly add a flow sensor from our industrial FS series to the F-OEM without the need of an additional board. Expand your system up to 32 outlets with 4 modules. Use the P-SWITCH buttons to quickly commute outlets between the two supplied pressure(s)/vacuum(s) and focus on the experiment. With a compact and modular design, the Push-Pull allows you to set and benefit quickly from the pressure-based flow control advantages for your experiments. Along with the MCFS-EZ or MCFS-EX, a Manifold can be added to redirect the pressure to multiple fluid reservoirs. Extension slots available, Pressure: 25 mbar (0.36 psi) / 69 mbar (0.9 psi) / 345 mbar (5 psi) / 1000 mbar (14.5 psi) /, USB cable, domino, electrical wires, 4 mm and 6 mm pneumatic tubing (4 m), Power supply 108 W if using a Switch module, Positive pressure source with necessary tubing and fittings, Negative pressure source with necessary tubing and fittings, Pressure regulator if pressure of different ranges are used. & Lourdes, Basabe-Desmonts Fernando, B.-L. Microfluidics and materials for smart water monitoring: A review. Flow sensors are connected for flow rate monitoring and control. It provides the best response time, pulseless, and highly stable flow conditions for high precision industrial microfluidic applications. What I like most is that you are independent of a computer and can directly control both positive and negative pressure.. When combined with theLINKmodule, the Flow EZcapabilities are extended by using Fluigent software to control the system, or to generate time based protocols and reccord data(OxyGEN software). The MFCSTM series and Flow EZTM with Fluigent valves can be used for automated cell perfusion as in the next video. 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, Success story of SEED Biosciences : Single-cell injection and impedance analysis, University of Cambridge: Giant unilamellar vesicle production and testing, The Micro/Nano Bioelectronics and Biosensors (MBIOS) from Tianjin University, Microbiome culture in droplet using dsurf surfactant, Droplet and particle manipulation using electrophoretic flow control, Cartilage on chip using Fluigent MFCS pressure controller, Fluid recirculation for cell perfusion with reduced shear stress, Droplet generation using syringe pumps and pressure-based flow controllers, Extended capabilities of pressure driven flow for microfluidics applications, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, Highly monodispersed droplet size No oscillation, No transitory regime Reagent saving Stop-flows, Wide range of volume can be controlled No compromise between volume and stability, Any mix or pressure ranges are available Highly customizable, Female luer lock (-800 to 2000 mbar) 4mm OD tube (7 bar), Dry, oil-free gas, air, any non corrosive or non explosive gas, Aqueous solvent, oil, organic solent, biological sample, Control in real-time, protocol automation, data record and export. is well-suited technology for applications that require mobile or compact fluidic control devices, including point of care (PoC) diagnostic and testing, or for water and soil on-site analysis. The P-SWITCH allows to multiply the outlets of the system and the SWITCH EZ controls microfluidic valves. Combine it with a flow sensor to regulate directly in flow rate and inject precise amounts of volume in the system.
When connected to a computer, one can use our Fluigent software tobenefit from automationorlive monitoring and recording of data. 6). The benefits of this technology are listed below. Janire, S., Raquel, C.-C., R_oisn, M. O. With the Flow EZ or Push-Pull modules one can precisely regulate and control pressure & vacuum, the LINK and LINK COM modules provide communication to a computer or any external instrument using TTL ports, USB cable or Serial port communication. Single gateway for industrial-grade microfluidic control: The platform can be used to control a pressure source and power other third-party devices required in the microfluidic system. Video courtesy of Pr Satoshi Sawai ( University of Tokyo, Japan). If you are seeking to replace high-precision syringe pumps or other conventional instruments, we offer modern microfluidic systems and components that enhance productivity.. Either 4 or 8 channels are available with different pressure ranges for microfluidic experiments. However, adding such leakage components influence the overall performance: the maximum pressure that can be reached has now decreased of about 15% (from 375 mbar to 310 mbar illustrated in the graph Fig. The algorithm includes a continuous optimization of the parameters that allow it to adapt to the interactions between microfluidic channels in complex situations. Inject and switch different flow paths. Pressure modules (positive pressure, negative pressure, or push-pull modules), microfluidic valve modules, and flow sensors are directly connected to the main platform.
Connecting the pressure outputs to airtight reservoirs provides precise and smooth control of the sample flow into the microfluidic device. It is at the heart of the Fluigents next generation performance providing the fastest, most stable and compact system for microfluidics available. The device is well designed and allows for easy control of my microfluidic chips. The MFCS-EX provides up to 8 negative or positive pressure channels to pressurize multiple fluid reservoirs. 8, 114 (2021). The pressure automatically adjusts in the background to maintain the set flow rate.
The F-OEM makes use of the best combination of pneumatic, mechanical, and electrical elements associated with our new generation algorithm: Fluigent Direct Flow-rate Control (DFC) a self-learning algorithm that performs continuous adjustment over the algorithm parameters based on the actual response time. TheFlow EZis the most advanced system available forpressure-based flow control. The platform allows one to choose the number of pressure modules (with different ranges, if required), valve modules, and flow sensors. The pressure automatically adjusts in the background to maintain the set flow rate. For flow-rate monitoring and control. Complex pressure and flow distribution can be eliminated as the unit can be placed at the location where pressure, vacuum, or flow control is needed. 0.1% thanks to our field-proven, patented FASTAB technology allowing optimal flow control with the robustness required in demanding industrial environments. Customize your own controller to answer your control needs. 1186, (2021). Graphically monitor in real-time the pressure measurement using the OxyGEN software. It is a standalone, modular platform that will perform complex fluidic operations. Fluigents i (patent pending) powerful pressure regulation algorithm is based on physical equations and self-learning routines that offer several benefits: By directly connecting a flow rate sensor, it is possible to monitor or control flow rate in real time.
Weighing in less than 170 g and with a volume of 140 cm3, Excellent stability and response time thanks to Fluigent pressure and liquid handling expertise. In this stand-alone configuration, the device allows for pressure or flow rate control and volume dispense making it ideal for benchtop use. Connect the outlets of the P-SWITCH directly to quake or on-chip pneumatic valves to actuate them. This allows the user to set a flow rate directly on the instrument display. ThePRESSURE UNITis a stand-alone sensor forcontinuous measurementof the pressure in a fluidic path. Configuration 2: 2 pressure modules 2 flow sensors. The F-OEM range of modules allows for regulation of vacuum/pressure down to -800 mbar and up to 7 bar, with the possibility to use a push-pull module (-800 / +1 000 mbar). It is well established that pressure-based flow control offers unequaled flow stability and response times. Support reservoir sizes from 2 mL to one liter laboratory bottles. It provides a dual interface USB and RS232 for high versatility in the integration. No flow sensors. Based on our industry leading experience, Fluigent has developed the patented QuadCore pneumatic system, the most advanced microfluidic pumping technology available. Continuous monitoring of water resources such as freshwater, seawater, and, in particular, wastewater and drinking water, for human and animal consumption, is essential2. As the equipment is not easily portable, samples can be compromised during travel. Our technology is an excellent fit for such applications as it is fully connected and provides excellent fluidic performance while being compact. Supports both pressure control and direct volume flow rate control depending on user needs. A new generation of point of care diagnostic devices has been recently developed for providing higher sensitivity diagnostics, such as nucleic acid amplification tests. These can easily be combined tomatch all application requirementsor reconfigure the system for a new experimental design. The use of pressure allows for a quick response time as well. The Push-Pull allows for regulatation of pressure and vacuum down to -800 mbar and up to 1 bar from one single channel. These can easily be combined tomatch all application requirementsor reconfigure the system for a new experimental design. https://cordis.europa.eu/article/id/421573-microfluidics-is-the-future-of-agriculture-with-on-site-soil-analysis, Development of complete device based on your needs, 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, (Patent pending) compact all-in-one pressure supply & pressure/flow control, Things you should know when integrating fluidics into your system, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, All in one micropump for pressure source and control, Need to be connected to an external pressure source and power supply, Pressure: Up to 600 mbar (for a single pump), No overshoot/undershoot, allowing for an immediate highly accurate and stable flow, Useful over a wide pressure or vacuum range (up to 600 mbar, -400 mbar vacuum, standalone pressure/vacuum regulation capability). It allows optimal flow control with the reliability required for demanding industrial environments. These can easily be joined together tomatch all application requirementsor reconfigure the system for a new experimental design. You can choose the number of channels of the device (4 or 8), each channel range from -800 mbar to 7 bar, and you even have the possibility to integrate a pressure or vacuum source inside the instrument. The PRESSURE UNIT product line allow for the accurate measurement of pressure and vaccum within the range -1000 mbar to 7 bar. The Push-Pull integrates the all-new DFC (Direct Flow Control) algorithm. Most pressure controllers require a pressure source which is regulated. 5 that compares Fluigent solution with a standalone gas micropump. Due to its individually stackable channels adapted for different pressures, the PX series allowed for control of the different pressure variations required. 3 pressure modules consisting of two 0 2000 mbar and a -800 + 1000 mbar are connected to the main F-OEM board for pressurizing two fluidic reservoirs, allowing for pressure-based fluid handling. 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, Key indicators to ensure long-term performance of your OEM flow control components, Things you should know when integrating fluidics into your system, DFC, self-learning flow rate control algorithm, Peristaltic pump vs pressure-based microfluidic flow control systems for Organ on-chip applications, Droplet generation using syringe pumps and pressure-based flow controllers, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, Pressure : 0 to 7000 mbar (101 psi) required pressure supply 7100 mbar (103 psi), Pressure: 0 to 2000 mbar (29 psi) required pressure supply 2100 mbar (30.4 psi) min, 2400 max, Pressure: 0 to 1000 mbar (14.5 psi) required pressure supply 1100 mbar (16 psi) min, 1300 max, Pressure: 0 to 345 mbar (5 psi) required pressure supply 1100 mbar (16 psi) (600 min, 1300 max), Pressure: 0 to 69 mbar (0.9 psi) required pressure supply 150 mbar (2.18 psi), Pressure: 0 to 25 mbar (0.36 psi) required pressure supply 150 mbar (2.18 psi), Vacuum : -25 mbar (-0.36 psi) / -69 mbar (-0.9 psi) / -345 mbar (-5 psi) / -800 mbar required vacuum -800 mbar (-11.6 psi), Push-Pull : -800 mbar (-11.6 psi) to 1000 mbar (14.5 psi) required pressure 1100 mbar (16 psi) min and 1300 max and vacuum -800 mbar (-11.6 psi) (min), 0.1% of full scale CV (on measured values), Liquid flow rate sensor input, customizable to work with third party sensors (through SDK), Control up to 4 x switches or valves per module compatible with Fluigent 2 position switches and rotary valves (M-switch, L-switch, 2-switch), Pressurized or bottled clean dry and non-corrosive or explosive gas (Ambient air, N2, Ar, CO2) (02 could be a thing, need to check), OD 4mm female push in fitting (on standard version, can be fittingless, then it is an M5 thread), >1W groundable for F-OEM platform (can be increased depending on peripherals, eg pump, fans etc) 6W maximum per FEZ module, 48W maximum per SWEZ module (rotary valves ~ 12W), External power, 2A or 7A available from standard, but custom can be accepted using MBPT terminal block connection, USB (standard board), RS232 (alternative board) + other protocols on demand, RJ45 female (or 2wire terminal block for SWEZ lite), 0-24VDC, digital controlled output 5 or 24 volts selectable, 2 x USB2.0 ports (available only on the USB connection protocol version, 0.1 Kg (F-OEM platform), 0.4 Kg (per pressure module with manifold), 0.3 kg (per switch module), Main electronic board. By providing two pressure sources (any vacuum or pressurefrom -800 to +2000 mbar), each module is able to deliver one of the two provided pressures through8 independent outlets. Bioeng. For more information or a technical discussion, 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics. The set-up allows us to further advance our research in both continuous flow and droplet microfluidics.. The flow generated can be measured or controlled with FLOW UNITs and the Flowboard. The Flow EZ integrates the all-new DFC (Direct Flow Control) algorithm. Using this engineered solution, pressure drop takes less than a second, and the maximum pressure reachable has not deteriorated (see graph). The MFCS is a pressure based microfluidic flow controller. The applied pressure will automatically adjust to maintain the flow rate. It offers excellent performance. In this stand-alone configuration, the device allows for pressure or flow rate control and volume dispense making it ideal for benchtop use. A low response time allows one to quicky execute operations such as stop flow and pressure/flow rate steps.