What can microfluidics do for microbiome research? Enzyme and Microbial Technology, 2003, 33(5): 569-575. This study is a comprehensive evaluation of the effects of various topographical cues on arterial- or venous-specific EC phenotype and function.
Herein, we developed a cost-effective kit to build a micro-peristaltic pump (~ 50 USD) consisting of 3D-printed and off-the-shelf components. and Yu, H., 2008. Moores Law is alive and well. However, quantification of cell viability in dense 3D tissue constructs is not straightforward. Published by Elsevier Ltd. https://doi.org/10.1016/j.ohx.2021.e00202. Featured in RSCs Chemical Biology, 2007, Issue 4Featured in RSCs Chemical Biology, 2007, Issue 4. Choudhury, Y.#, Toh, Y.C.#, Xing, J., Qu, Y., Poh, J., Huan, L., Tan, H.S., Kanesvaran, R., Yu, H. and Tan, M.H., 2017. Michinao Hashimoto is an Associate Professor at Singapore University of Technology and Design leading Soft Fluidics Group. Scalable cell alignment on optical media substrates, 2013,Biomaterials,34(21): 5078-5087, Wang, Y.,Toh, Y.C., Li, Q., Nugraha, B., Zheng, B., Lu, T.B., Gao, Y., Ng, M.M.L. They developed a highly-customizable, 3D-printed peristaltic pump kit.
A multiplex microfluidic platform to systematically investigate the dosetime shear responses on hPSCEC morphology and arterialvenous phenotypes over a range of magnitudes coincidental with physiological levels of embryonic and adult vasculatures. Akshaya Srinivasan, Yi-Chin Toh*, Human Pluripotent Stem Cell-derived Neural Crest Cells for Tissue Regeneration and Disease Modeling, 2019, Frontiers in Molecular Neuroscience, 12, https://doi.org/10.3389/fnmol.2019.00039, This paper reviews the various in vitro strategies that have been used to derive NCCs from hPSCs and specify NCCs into cranial, trunk and vagal subpopulations and their derivatives. From a 4-bit CPU that clocked a whopping 740 kilohertz, capable of executing 92,000 instructions per second and accessing 4KB of program memory and 640 bytes of RAM to a a chip with up to 16 cores and 24 threads, 5.2 gigahertz turbo frequency, 30MB shared Intel Smart Cache, DDR5 memory and both PCIe 4.0 and 5.0 for the latest storage and high-performance discrete graphics (Intel 12th Gen) 2005,Biomaterials,26(19): 41494160. By applying the modularized approach, 2D and 3D networks of microchannels with high complexity (i.e., multiple branching networks) and small crosssectional dimensions (w=75m and h=100m) are fabricated in photoresins and hydrogels. The journey of how circuits are transferred on silicon wafers. She worked on the synthesizing and characterizing of Chitosan/Diopside scaffolds and their applications as Bone Tissue Engineering substrates. Seep Arora, Jing Ying Adele Lam, Christine Cheung, Evelyn KF Yim, Yi-Chin Toh*, Microtechnologies for Experimental Stem Cell Biology, Highly-customizable 3D-printed peristaltic pump kit, A micropatterned human-specific neuroepithelial tissue for modeling gene and drug-induced neurodevelopmental defects, Comparative Craniofacial Bone Regeneration Capacities of Mesenchymal Stem Cells Derived from Human Neural Crest Stem Cells and Bone Marrow, Acrylated Epoxidized Soybean Oil / Hydroxyapatite-Based Nanocomposite Scaffolds Prepared by Additive Manufacturing for Bone Tissue Engineering. Fluid shear stress primes mouse embryonic stem cells for differentiation in a self-renewing environment via heparan sulfate proteoglycans transduction, 2011,FASEB Journal, 25: 1208-1217, Toh, Y.C., Blagovi, K. and Voldman, J., 2010. Geetika Sahni, Shu-Yung Chang1, Jeremy Teo Choon Meng, Jerome Zu Yao Tan, Jean Jacques Clement Fatien, Carine Bonnard, Kagistia Hana Utami, Puck Wee Chan, Thong Teck Tan, Umut Altunoglu, Hlya Kayserili, Mahmoud Pouladi, Bruno Reversade, Yi-Chin Toh*, A micropatterned human-specific neuroepithelial tissue for modeling gene and drug-induced neurodevelopmental defects, 2021, Advanced Science, https://doi.org/10.1002/advs.202001100. Different endothelial cell subtypes often exhibit similar morphological response to topographical cues, however, they can demonstrate different phenotypic and functional responses. Tasnim, F., Toh, Y.C., Qu, Y., Li, H., Phan, D., Narmada, B.C., Ananthanarayanan, A., Mittal, N., Meng, R.Q. and Yu, H., 2013. She obtained her B.Eng in Chemical Engineering and Ph.D in Bioengineering from the National University of Singapore in 2001 and 2008 respectively. We also discovered that substrate stiffness modulated the differentiation of NCSCs to eMSCs via a Rho-ROCK dependent pathway involving CD44-mediated inhibition of PDGFR signaling. 2016,Molecular Pharmaceutics,13(6), pp.1947-1957. He has authored over 400 peer-reviewed journal papers and delivered more than 390 plenary/keynote/invited talks. I want to present my gratitude to IBM for arranging such a fantastic workshop. Lor Huai Chong, Huan Li, Isaac Wetzel, Hansang Cho, Yi-Chin Toh*, A liver-immune coculture array for predicting systemic drug-induced skin sensitization, 2018, Lab on a Chip 18: 3239. Sahni, G.,Yuan, J.andToh, Y.C. Her thesis focused on understanding the physical and chemical properties of biodegradable polyesters and their applications as protein delivery systems. He is currently a Ph.D. and Yu, H., 2013. Y.C. degrees in Chemistry and Biochemistry/Biophysics from Oregon State University (2003), and Ph.D. degree from Harvard University (2009), followed by postdoctoral training at Massachusetts Institute of Technology and Childrens Hospital Boston. and Yu, H., 2008. A microfluidic 3D hepatocyte chip for drug toxicity testing, 2009,Lab on a Chip, 9(14): 2026-2035, Zhang, C., Chia, S.M., Ong, S.M., Zhang, S.,Toh, Y.C., van Noort, D. and Yu, H., 2009. Toh, Y.C., Ho, S.T., Zhou, Y., Hutmacher, D.W. and Yu, H., 2005.
Dendrimer hydrazides asmultivalent transient inter cellular linkers, Biomaterials, 2008, 29 (27): 3693-3702, Ong, S.M., Zhang, C.,Toh, Y.C., Kim, S.H., Foo, H.L., Tan, C.H., van Noort, D., Park, S. and Yu, H., 2008. *, 2016. In this paper, we combine hPSC micropatterning and a temporallysequenced induction protocol to specify neuroepithelial (NE) cells in spatial juxtaposition to mesoendoderm cells. nine Louis Jun Ye Ong, Liang Zhu, Gabriel Jenn Sern Tan,Yi-Chin Toh*, Quantitative Image-Based Cell Viability (QuantICV) Assay for Microfluidic 3D Tissue Culture Applications, 2020,Micromachines, 11 (7): 10.3390/mi11070669. Cellular responses to a nanofibrous environment, 2006,NanoToday, 1(3): 34-43. Download : Download high-res image (220KB)Download : Download full-size image. To demonstrate the forward compatibility, we developed a flowrate calibration tool that was readily interfaced with the developed pump system. *,A pump-free microfluidic 3D perfusion platform for the efficient differentiation of human hepatocyte-like cells, 2017,Bioengineering & Biotechnology. Tasnim, F., Phan, D., Toh, Y.C. He has been also awarded with theZwick Science Awardfor his studies on Mechanical Testing, and best PhD thesis at the University of Barcelona for studies in the use of biopolymers inMicroelectronicsandBiomedicine. In 2020, he joined Shrike Zhangs Laboratory at Harvard Medical School as a visiting research trainee, working on light-based 3D bioprinting. It took me approx 2 hrs to complete, but finally, I found the answer. Xing, J.,Toh, Y.C., Xu, S. and Yu, H., 2015. The SHAKTI free and open source silicon project has reached another milestone with the boot-up of the Moushik, an Arduino-compatible SoC. *, Substrate stiffness modulates the multipotency of neural crest derived human ectomesenchymal stem cells via CD44 mediated PDGFR signaling, 2018,Biomaterials167:153-167. and Yu, H., 2007. Toh, Y.C., Xing, J. and Yu, H., 2015. Development of poly (lactic-co-glycolic acid)-collagen scaffolds for tissue engineering. Javier regularly collaborates with some of the most important international media outlets. Michinao received his B.S. Jyothsna joined The Fermart Lab in 2016 as NUS-SUTD PhD fellow. We use cookies to help provide and enhance our service and tailor content and ads. Toh, Y.C., Ng, S., Khong, Y.M., Zhang, X., Zhu, Y., Lin, P.C., Te, C.M., Sun, W. and Yu, H., 2006. It amused me to know about how the graphics and programs were squeezed in 16KB ROM cartridge and executed by an 8bit processor with just 3 internal resistors (Accumulator, X, Y) a 256 Bytes of RAM (127 Bytes of RAM for Variables & 127 Bytes shared with Television Adaptor Video memory) It was very interesting and I had learned a lot about old ROM Programming, Sprits design, 6507 CPU chip, etc. Later, he moved to the Wyss Institute at Harvard University, where he designed and made Shrilk, a compostable and biocompatible material inspired by the insect cuticle. 2007,Lab on a Chip, 7(3): 302-309. His other hobbies include soccer, table tennis, and graphics design. He is also an elected member of the World Council of Biomechanics. *,A 3D Printed Microfluidic Perfusion Device for Multicellular Spheroid Cultures, 2017,Biofabrication, Ong, L.J.Y., Chong, L.H., Jin, L., Singh, P.K., Lee, P.S., Yu, H., Ananthanarayanan, A., Leo, H.L.,Toh, Y.C. Akshaya Srinivasan, Nelson Teo, Poon Kei Jun, Priya Tiwari, Akhilandeshwari Ravichandran, Wen Feng, Swee Hin Teoh, Thiam Chye Lim, Yi-Chin Toh*, Comparative Craniofacial Bone Regeneration Capacities of Mesenchymal Stem Cells Derived from Human Neural Crest Stem Cells and Bone Marrow, 2021, ACS Biomaterial Science and Research, 7, 1, 207221. Bob Noyces integrated circuits and those 4KB took us to the moon. Khong, S. Chang, V.D. Semiconductor Technology. While miniature pumps are commercially available, they require proprietary and expensive control systems. Hsih-Yin Tan received her PhD from Micro- and Nanotechnology Department (current Department of Health Technology from Denmarks Tekniske Universitet, Denmark in 2016. Youve made so much possible over the past half-century. Functionally enhanced human stem cell derived hepatocytes in galactosylated cellulosic sponges for hepatotoxicity testing.
Kim, L.,Toh, Y.C., Voldman, J. and Yu, H., 2007. Biomicrofluidics, 2020, 14, (5), DOI: 10.1063/5.0012185. Using a modular design, the kit is easily customizable, easy to replace, and has exceptional forward compatibility. Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates. Seep, Evelyn Yim, Yi-Chin Toh*, Environmental specification of pluripotent stem cell derived endothelial cells towards arterial and venous subtypes,2019, Frontiers in Bioengineering and Biotechnologyhttps://doi.org/10.3389/fbioe.2019.00143. A Practical Guide to Microfluidic Perfusion Culture of Adherent Mammalian Cells. Cost-effective differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules. This paper discusses how microfluidic technologies can value add to the different phases of human microbiome research as well as the unique challenges that microfluidics must overcome when working with microbiome-relevant biological materials. Currently, his groups research directions include 3D printing and bioprinting for microfluidic devices, low-cost instrumentation, flexible devices, and 3D food printing. He is also the Director of the Institute for Health Innovation and Technology (iHealthtech) and Founding Director of the Singapore Health Technologies Consortium. The pump is designed in the form of a kit, allowing end-users to customize the setup. You will receive a link to create a new password via email.
The pump is powered and controlled by Arduino, an open-source electronics platform. Dibakar Mondal, Akshaya Srinivasan, Patricia Comeau, Yi-Chin Toh*, Thomas L Willett, Acrylated Epoxidized Soybean Oil / Hydroxyapatite-Based Nanocomposite Scaffolds Prepared by Additive Manufacturing for Bone Tissue Engineering, 2021, Materials Science & Engineering C, 118:111400. | How does your Smartphone store data?
2021 The Authors. *, Leo, H.L. The pumps exhibited good repeatability in flowrates and functioned inside a cell incubator (at 37C and 95 % humidity) for seven days without noticeable issues in the performance. Before joining QUT, she led an independent research group as an Assistant Professor at the Department of Biomedical Engineering, National University of Singapore. This review aims to provide a framework as well as highlight opportunities to advance pluripotent stem cell endothelial cell differentiation protocols from EC lineage commitment to arterial-venous specification. Louis Jun Ye Ong, Terry Tsz Him Ching, Lor Huai Chong, Seep Arora, Huan Li, Ramanuj DasGupta, Po Ki Yuen, Yi-Chin Toh*, Self-Aligning Tetris-Like (TILE) Modular Microfluidic Platform for Mimicking Multi-Organ Interactions, 2019, Lab on a Chip,19, 2178-2191, 10.1039/C9LC00160C. This paper demonstrated that substratestiffness mediated mechanical cues can directly modulate the development of ectodermal MSCs(eMSCs) from a precursor human neural crest stem cell (NCSC) population.
She is passionate about understanding the properties of different types of materials and how they can be applied to the domain of living or biological systems. Copyright 2022 Elsevier B.V. or its licensors or contributors. Application of polyelectrolyte complex coacervation to improve the seeding efficiency of bone marrow stromal cells in 3D scaffolds. Being Restless and curious, I invested my time and solved it. A Configurable 3D Microenvironment in a Micro-fluidic Channel forPrimary Hepatocytes Culture. Wen, F., Chang, S.,Toh, Y.C., Teoh, S.H. His opinions and views on scientific issues and environmental policies have been, for example, covered in personal interviews, documentaries, and articles of National Geographic, BBC, PBS, FOX, Euronews, Discovery Channel, The Guardian, Huffington Post, Scientific American. and Yu, H., 2013. 2016,JoVE (Journal of Visualized Experiments), 112: pp.e54097-e54097. Advancing Stem Cell Research with microtechnologies: opportunities and challenges, 2010,Integrative Biology, 2: 305-325, Toh, Y.C., Lim, T.C., Tai, D., Xiao, G., van Noort, D. and Yu, H., 2009. ScienceDirect is a registered trademark of Elsevier B.V. ScienceDirect is a registered trademark of Elsevier B.V. Highly-customizable 3D-printed peristaltic pump kit. Please enter your username or email address. Scientific Reports,7. You can contribute your articles on emerging fields toPioneering Minds. He and his team have garnered close to 100 research awards and honors including Highly Cited Researcher 2019, Winner of IDTechEx Launchpad 2017, International Precision Medicine Conference Prize 2017, ASEAN Outstanding Engineering Achievement Award and Asian Scientists 100 in 2016, Vladimir K. Zworykin Award in 2015, Universitys Outstanding Researcher Award and Outstanding Innovator Award in 2014, the Credit Suisse Technopreneur of the Year Award, Wall Street Journal Asian Innovation Award (Gold) in 2012, Presidents Technology Award in 2011 and the IES Prestigious Engineering Achievement Award in 2010 among others. Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation, 2015,Biomaterials,50 (0): 87-97. Lor Huai Chong, Celine Ng, Huan Li, Edmund Feng Tian, Abhishek Ananthanarayanan, Michael McMillian, Yi-Chin Toh, Hepatic Bioactivation of Skin-Sensitizing Drugs to Immunogenic Reactive Metabolites, 2019,ACS Omega,4, 9, 13902-13912. Today I came across a new course Assembly Language by Making Games for Atari 2600 Professor Lim is the inaugural NUSS chair Professor at the Department of Biomedical Engineering and Founding Principal Investigator at the Mechanobiology Institute. Integrating sensitive quantification of hepatic metabolic functions by capillary electrophoresis with laser-induced fluorescence detection, 2008,Analyst, 133: 326-330, Wen, F., Chang, S.,Toh, Y.C., Arooz, T., Zhuo, L., Teoh, S.H. Today my grandkids can explore the universe from a handheld computer. She is also an aspiring photographer. A gel-free 3D microfluidic cell culture system, 2008,Biomaterials, 29(22): 3237-3244, Toh, Y.C., Zhang, J., Khong, Y.M., Du, Y., Sun, W. and Yu, H., 2008. 2D And 3D Combo Could Keep Moores Law Going. We also discuss the potential applications of these human specific NCC platforms, including the use of iPSCs for disease modeling and the potential of NCCs for future regenerative applications, Seep Arora, Jing Ying Adele Lam, Christine Cheung, Evelyn KF Yim, Yi-Chin Toh*, Determination of critical shear stress for maturation of human pluripotent stem cell derived endothelial cells towards an arterial subtype, 2019, Biotechnology and Bioengineering, 116 (5), 1164-1175, https://doi.org/10.1002/bit.26910. This paper demonstrates the generation and quantification of immunogenic metabolite species of paradigm skin sensitising drugs using a human progenitor cell (HepaRG)-derived hepatocyte model and highly sensitive liquid chromatography-mass spectrometry analytical assays.
The peristaltic pump kit exhibited good repeatability in flow rates and functioned for 7 days without any noticeable issues in performance. To make microfluidic pumps more accessible to the scientific community, Singapore University of Technology and Design (SUTD) researchers have developed a highly-customizable and Arduino-powered peristaltic pump kit. Her research interest is in engineering multi-scale tissue models to mimic complex biological interactions during human development and diseases, as well as translating them into scalable platforms for disease modeling and drug testing applications. Featured in Global Medical Discovery(ISSN 1929-8536),Featured in Global Medical Discovery (ISSN 1929-8536), Jan 2013, Toh, Y.C., Blagovic, K., Yu, H. and Voldman, J., 2011. By evaluating NE tissue structural dysmorphia in the micropatterned NE model, we can successfully model gene- and drug-induced neurodevelopmental defects. Dr Toh is a recipient of the Australia Research Council Future Fellowship, National University of Singapore Research Scholarship, A*STAR Graduate Scholarship and A*STAR International Fellowship. 2005,Assay and Drug Development Technologies,3(2): 169-176. Seep Arora, Christine Cheung, Evelyn Yim*, Yi-Chin Toh*, Topography elicits distinct phenotypes and functions in human primary and stem cell derived endothelial cells, 2020, Biomaterials, 234, 119747. This kit was designed with modular components (i.e., each component followed a standardized unit) to achieve (1) customizability (users can easily reconfigure various components to comply with their experiments), (2) forward compatibility (new parts with the standardized unit can be designed and easily interfaced to the current kit), and (3) easy replacement of the parts experiencing wear and tear. By continuing you agree to the use of cookies. Xianzhu Zhang, Dandan Cai, Feifei Zhou, Jie Yu, Xinyu Wu, Dongsheng Yu, Yiwei Zou, Yi Hong, Chunhui Yuan, Yishan Chen, Zongyou Pan, Varitsara Bunpetch, Heng Sun, Chengrui An, Yi-Chin Toh, Hongwei Ouyang, Shufang Zhang, Targeting downstream subcellular YAP activity as a function of matrix stiness with Verteporn-encapsulated chitosan microsphere attenuates osteoarthritis, 2020,Biomaterials, 232: 119724, Terry Ching, Yi-Chin Toh, Michinao Hashimoto, Fabrication of Complex 3D Fluidic Networks via Modularized Stereolithography, 2019, Advanced Engineering Materials, 1901109, https://doi.org/10.1002/adem.201901109.
Herein, we developed a cost-effective kit to build a micro-peristaltic pump (~ 50 USD) consisting of 3D-printed and off-the-shelf components. and Yu, H., 2008. Moores Law is alive and well. However, quantification of cell viability in dense 3D tissue constructs is not straightforward. Published by Elsevier Ltd. https://doi.org/10.1016/j.ohx.2021.e00202. Featured in RSCs Chemical Biology, 2007, Issue 4Featured in RSCs Chemical Biology, 2007, Issue 4. Choudhury, Y.#, Toh, Y.C.#, Xing, J., Qu, Y., Poh, J., Huan, L., Tan, H.S., Kanesvaran, R., Yu, H. and Tan, M.H., 2017. Michinao Hashimoto is an Associate Professor at Singapore University of Technology and Design leading Soft Fluidics Group. Scalable cell alignment on optical media substrates, 2013,Biomaterials,34(21): 5078-5087, Wang, Y.,Toh, Y.C., Li, Q., Nugraha, B., Zheng, B., Lu, T.B., Gao, Y., Ng, M.M.L. They developed a highly-customizable, 3D-printed peristaltic pump kit.
A multiplex microfluidic platform to systematically investigate the dosetime shear responses on hPSCEC morphology and arterialvenous phenotypes over a range of magnitudes coincidental with physiological levels of embryonic and adult vasculatures. Akshaya Srinivasan, Yi-Chin Toh*, Human Pluripotent Stem Cell-derived Neural Crest Cells for Tissue Regeneration and Disease Modeling, 2019, Frontiers in Molecular Neuroscience, 12, https://doi.org/10.3389/fnmol.2019.00039, This paper reviews the various in vitro strategies that have been used to derive NCCs from hPSCs and specify NCCs into cranial, trunk and vagal subpopulations and their derivatives. From a 4-bit CPU that clocked a whopping 740 kilohertz, capable of executing 92,000 instructions per second and accessing 4KB of program memory and 640 bytes of RAM to a a chip with up to 16 cores and 24 threads, 5.2 gigahertz turbo frequency, 30MB shared Intel Smart Cache, DDR5 memory and both PCIe 4.0 and 5.0 for the latest storage and high-performance discrete graphics (Intel 12th Gen) 2005,Biomaterials,26(19): 41494160. By applying the modularized approach, 2D and 3D networks of microchannels with high complexity (i.e., multiple branching networks) and small crosssectional dimensions (w=75m and h=100m) are fabricated in photoresins and hydrogels. The journey of how circuits are transferred on silicon wafers. She worked on the synthesizing and characterizing of Chitosan/Diopside scaffolds and their applications as Bone Tissue Engineering substrates. Seep Arora, Jing Ying Adele Lam, Christine Cheung, Evelyn KF Yim, Yi-Chin Toh*, Microtechnologies for Experimental Stem Cell Biology, Highly-customizable 3D-printed peristaltic pump kit, A micropatterned human-specific neuroepithelial tissue for modeling gene and drug-induced neurodevelopmental defects, Comparative Craniofacial Bone Regeneration Capacities of Mesenchymal Stem Cells Derived from Human Neural Crest Stem Cells and Bone Marrow, Acrylated Epoxidized Soybean Oil / Hydroxyapatite-Based Nanocomposite Scaffolds Prepared by Additive Manufacturing for Bone Tissue Engineering. Fluid shear stress primes mouse embryonic stem cells for differentiation in a self-renewing environment via heparan sulfate proteoglycans transduction, 2011,FASEB Journal, 25: 1208-1217, Toh, Y.C., Blagovi, K. and Voldman, J., 2010. Geetika Sahni, Shu-Yung Chang1, Jeremy Teo Choon Meng, Jerome Zu Yao Tan, Jean Jacques Clement Fatien, Carine Bonnard, Kagistia Hana Utami, Puck Wee Chan, Thong Teck Tan, Umut Altunoglu, Hlya Kayserili, Mahmoud Pouladi, Bruno Reversade, Yi-Chin Toh*, A micropatterned human-specific neuroepithelial tissue for modeling gene and drug-induced neurodevelopmental defects, 2021, Advanced Science, https://doi.org/10.1002/advs.202001100. Different endothelial cell subtypes often exhibit similar morphological response to topographical cues, however, they can demonstrate different phenotypic and functional responses. Tasnim, F., Toh, Y.C., Qu, Y., Li, H., Phan, D., Narmada, B.C., Ananthanarayanan, A., Mittal, N., Meng, R.Q. and Yu, H., 2013. She obtained her B.Eng in Chemical Engineering and Ph.D in Bioengineering from the National University of Singapore in 2001 and 2008 respectively. We also discovered that substrate stiffness modulated the differentiation of NCSCs to eMSCs via a Rho-ROCK dependent pathway involving CD44-mediated inhibition of PDGFR signaling. 2016,Molecular Pharmaceutics,13(6), pp.1947-1957. He has authored over 400 peer-reviewed journal papers and delivered more than 390 plenary/keynote/invited talks. I want to present my gratitude to IBM for arranging such a fantastic workshop. Lor Huai Chong, Huan Li, Isaac Wetzel, Hansang Cho, Yi-Chin Toh*, A liver-immune coculture array for predicting systemic drug-induced skin sensitization, 2018, Lab on a Chip 18: 3239. Sahni, G.,Yuan, J.andToh, Y.C. Her thesis focused on understanding the physical and chemical properties of biodegradable polyesters and their applications as protein delivery systems. He is currently a Ph.D. and Yu, H., 2013. Y.C. degrees in Chemistry and Biochemistry/Biophysics from Oregon State University (2003), and Ph.D. degree from Harvard University (2009), followed by postdoctoral training at Massachusetts Institute of Technology and Childrens Hospital Boston. and Yu, H., 2008. A microfluidic 3D hepatocyte chip for drug toxicity testing, 2009,Lab on a Chip, 9(14): 2026-2035, Zhang, C., Chia, S.M., Ong, S.M., Zhang, S.,Toh, Y.C., van Noort, D. and Yu, H., 2009. Toh, Y.C., Ho, S.T., Zhou, Y., Hutmacher, D.W. and Yu, H., 2005. Dendrimer hydrazides asmultivalent transient inter cellular linkers, Biomaterials, 2008, 29 (27): 3693-3702, Ong, S.M., Zhang, C.,Toh, Y.C., Kim, S.H., Foo, H.L., Tan, C.H., van Noort, D., Park, S. and Yu, H., 2008. *, 2016. In this paper, we combine hPSC micropatterning and a temporallysequenced induction protocol to specify neuroepithelial (NE) cells in spatial juxtaposition to mesoendoderm cells. nine Louis Jun Ye Ong, Liang Zhu, Gabriel Jenn Sern Tan,Yi-Chin Toh*, Quantitative Image-Based Cell Viability (QuantICV) Assay for Microfluidic 3D Tissue Culture Applications, 2020,Micromachines, 11 (7): 10.3390/mi11070669. Cellular responses to a nanofibrous environment, 2006,NanoToday, 1(3): 34-43. Download : Download high-res image (220KB)Download : Download full-size image. To demonstrate the forward compatibility, we developed a flowrate calibration tool that was readily interfaced with the developed pump system. *,A pump-free microfluidic 3D perfusion platform for the efficient differentiation of human hepatocyte-like cells, 2017,Bioengineering & Biotechnology. Tasnim, F., Phan, D., Toh, Y.C. He has been also awarded with theZwick Science Awardfor his studies on Mechanical Testing, and best PhD thesis at the University of Barcelona for studies in the use of biopolymers inMicroelectronicsandBiomedicine. In 2020, he joined Shrike Zhangs Laboratory at Harvard Medical School as a visiting research trainee, working on light-based 3D bioprinting. It took me approx 2 hrs to complete, but finally, I found the answer. Xing, J.,Toh, Y.C., Xu, S. and Yu, H., 2015. The SHAKTI free and open source silicon project has reached another milestone with the boot-up of the Moushik, an Arduino-compatible SoC. *, Substrate stiffness modulates the multipotency of neural crest derived human ectomesenchymal stem cells via CD44 mediated PDGFR signaling, 2018,Biomaterials167:153-167. and Yu, H., 2007. Toh, Y.C., Xing, J. and Yu, H., 2015. Development of poly (lactic-co-glycolic acid)-collagen scaffolds for tissue engineering. Javier regularly collaborates with some of the most important international media outlets. Michinao received his B.S. Jyothsna joined The Fermart Lab in 2016 as NUS-SUTD PhD fellow. We use cookies to help provide and enhance our service and tailor content and ads. Toh, Y.C., Ng, S., Khong, Y.M., Zhang, X., Zhu, Y., Lin, P.C., Te, C.M., Sun, W. and Yu, H., 2006. It amused me to know about how the graphics and programs were squeezed in 16KB ROM cartridge and executed by an 8bit processor with just 3 internal resistors (Accumulator, X, Y) a 256 Bytes of RAM (127 Bytes of RAM for Variables & 127 Bytes shared with Television Adaptor Video memory) It was very interesting and I had learned a lot about old ROM Programming, Sprits design, 6507 CPU chip, etc. Later, he moved to the Wyss Institute at Harvard University, where he designed and made Shrilk, a compostable and biocompatible material inspired by the insect cuticle. 2007,Lab on a Chip, 7(3): 302-309. His other hobbies include soccer, table tennis, and graphics design. He is also an elected member of the World Council of Biomechanics. *,A 3D Printed Microfluidic Perfusion Device for Multicellular Spheroid Cultures, 2017,Biofabrication, Ong, L.J.Y., Chong, L.H., Jin, L., Singh, P.K., Lee, P.S., Yu, H., Ananthanarayanan, A., Leo, H.L.,Toh, Y.C. Akshaya Srinivasan, Nelson Teo, Poon Kei Jun, Priya Tiwari, Akhilandeshwari Ravichandran, Wen Feng, Swee Hin Teoh, Thiam Chye Lim, Yi-Chin Toh*, Comparative Craniofacial Bone Regeneration Capacities of Mesenchymal Stem Cells Derived from Human Neural Crest Stem Cells and Bone Marrow, 2021, ACS Biomaterial Science and Research, 7, 1, 207221. Bob Noyces integrated circuits and those 4KB took us to the moon. Khong, S. Chang, V.D. Semiconductor Technology. While miniature pumps are commercially available, they require proprietary and expensive control systems. Hsih-Yin Tan received her PhD from Micro- and Nanotechnology Department (current Department of Health Technology from Denmarks Tekniske Universitet, Denmark in 2016. Youve made so much possible over the past half-century. Functionally enhanced human stem cell derived hepatocytes in galactosylated cellulosic sponges for hepatotoxicity testing.
Kim, L.,Toh, Y.C., Voldman, J. and Yu, H., 2007. Biomicrofluidics, 2020, 14, (5), DOI: 10.1063/5.0012185. Using a modular design, the kit is easily customizable, easy to replace, and has exceptional forward compatibility. Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates. Seep, Evelyn Yim, Yi-Chin Toh*, Environmental specification of pluripotent stem cell derived endothelial cells towards arterial and venous subtypes,2019, Frontiers in Bioengineering and Biotechnologyhttps://doi.org/10.3389/fbioe.2019.00143. A Practical Guide to Microfluidic Perfusion Culture of Adherent Mammalian Cells. Cost-effective differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules. This paper discusses how microfluidic technologies can value add to the different phases of human microbiome research as well as the unique challenges that microfluidics must overcome when working with microbiome-relevant biological materials. Currently, his groups research directions include 3D printing and bioprinting for microfluidic devices, low-cost instrumentation, flexible devices, and 3D food printing. He is also the Director of the Institute for Health Innovation and Technology (iHealthtech) and Founding Director of the Singapore Health Technologies Consortium. The pump is designed in the form of a kit, allowing end-users to customize the setup. You will receive a link to create a new password via email. The pump is powered and controlled by Arduino, an open-source electronics platform. Dibakar Mondal, Akshaya Srinivasan, Patricia Comeau, Yi-Chin Toh*, Thomas L Willett, Acrylated Epoxidized Soybean Oil / Hydroxyapatite-Based Nanocomposite Scaffolds Prepared by Additive Manufacturing for Bone Tissue Engineering, 2021, Materials Science & Engineering C, 118:111400. | How does your Smartphone store data?
2021 The Authors. *, Leo, H.L. The pumps exhibited good repeatability in flowrates and functioned inside a cell incubator (at 37C and 95 % humidity) for seven days without noticeable issues in the performance. Before joining QUT, she led an independent research group as an Assistant Professor at the Department of Biomedical Engineering, National University of Singapore. This review aims to provide a framework as well as highlight opportunities to advance pluripotent stem cell endothelial cell differentiation protocols from EC lineage commitment to arterial-venous specification. Louis Jun Ye Ong, Terry Tsz Him Ching, Lor Huai Chong, Seep Arora, Huan Li, Ramanuj DasGupta, Po Ki Yuen, Yi-Chin Toh*, Self-Aligning Tetris-Like (TILE) Modular Microfluidic Platform for Mimicking Multi-Organ Interactions, 2019, Lab on a Chip,19, 2178-2191, 10.1039/C9LC00160C. This paper demonstrated that substratestiffness mediated mechanical cues can directly modulate the development of ectodermal MSCs(eMSCs) from a precursor human neural crest stem cell (NCSC) population.
She is passionate about understanding the properties of different types of materials and how they can be applied to the domain of living or biological systems. Copyright 2022 Elsevier B.V. or its licensors or contributors. Application of polyelectrolyte complex coacervation to improve the seeding efficiency of bone marrow stromal cells in 3D scaffolds. Being Restless and curious, I invested my time and solved it. A Configurable 3D Microenvironment in a Micro-fluidic Channel forPrimary Hepatocytes Culture. Wen, F., Chang, S.,Toh, Y.C., Teoh, S.H. His opinions and views on scientific issues and environmental policies have been, for example, covered in personal interviews, documentaries, and articles of National Geographic, BBC, PBS, FOX, Euronews, Discovery Channel, The Guardian, Huffington Post, Scientific American. and Yu, H., 2013. 2016,JoVE (Journal of Visualized Experiments), 112: pp.e54097-e54097. Advancing Stem Cell Research with microtechnologies: opportunities and challenges, 2010,Integrative Biology, 2: 305-325, Toh, Y.C., Lim, T.C., Tai, D., Xiao, G., van Noort, D. and Yu, H., 2009. ScienceDirect is a registered trademark of Elsevier B.V. ScienceDirect is a registered trademark of Elsevier B.V. Highly-customizable 3D-printed peristaltic pump kit. Please enter your username or email address. Scientific Reports,7. You can contribute your articles on emerging fields toPioneering Minds. He and his team have garnered close to 100 research awards and honors including Highly Cited Researcher 2019, Winner of IDTechEx Launchpad 2017, International Precision Medicine Conference Prize 2017, ASEAN Outstanding Engineering Achievement Award and Asian Scientists 100 in 2016, Vladimir K. Zworykin Award in 2015, Universitys Outstanding Researcher Award and Outstanding Innovator Award in 2014, the Credit Suisse Technopreneur of the Year Award, Wall Street Journal Asian Innovation Award (Gold) in 2012, Presidents Technology Award in 2011 and the IES Prestigious Engineering Achievement Award in 2010 among others. Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation, 2015,Biomaterials,50 (0): 87-97. Lor Huai Chong, Celine Ng, Huan Li, Edmund Feng Tian, Abhishek Ananthanarayanan, Michael McMillian, Yi-Chin Toh, Hepatic Bioactivation of Skin-Sensitizing Drugs to Immunogenic Reactive Metabolites, 2019,ACS Omega,4, 9, 13902-13912. Today I came across a new course Assembly Language by Making Games for Atari 2600 Professor Lim is the inaugural NUSS chair Professor at the Department of Biomedical Engineering and Founding Principal Investigator at the Mechanobiology Institute. Integrating sensitive quantification of hepatic metabolic functions by capillary electrophoresis with laser-induced fluorescence detection, 2008,Analyst, 133: 326-330, Wen, F., Chang, S.,Toh, Y.C., Arooz, T., Zhuo, L., Teoh, S.H. Today my grandkids can explore the universe from a handheld computer. She is also an aspiring photographer. A gel-free 3D microfluidic cell culture system, 2008,Biomaterials, 29(22): 3237-3244, Toh, Y.C., Zhang, J., Khong, Y.M., Du, Y., Sun, W. and Yu, H., 2008. 2D And 3D Combo Could Keep Moores Law Going. We also discuss the potential applications of these human specific NCC platforms, including the use of iPSCs for disease modeling and the potential of NCCs for future regenerative applications, Seep Arora, Jing Ying Adele Lam, Christine Cheung, Evelyn KF Yim, Yi-Chin Toh*, Determination of critical shear stress for maturation of human pluripotent stem cell derived endothelial cells towards an arterial subtype, 2019, Biotechnology and Bioengineering, 116 (5), 1164-1175, https://doi.org/10.1002/bit.26910. This paper demonstrates the generation and quantification of immunogenic metabolite species of paradigm skin sensitising drugs using a human progenitor cell (HepaRG)-derived hepatocyte model and highly sensitive liquid chromatography-mass spectrometry analytical assays.
The peristaltic pump kit exhibited good repeatability in flow rates and functioned for 7 days without any noticeable issues in performance. To make microfluidic pumps more accessible to the scientific community, Singapore University of Technology and Design (SUTD) researchers have developed a highly-customizable and Arduino-powered peristaltic pump kit. Her research interest is in engineering multi-scale tissue models to mimic complex biological interactions during human development and diseases, as well as translating them into scalable platforms for disease modeling and drug testing applications. Featured in Global Medical Discovery(ISSN 1929-8536),Featured in Global Medical Discovery (ISSN 1929-8536), Jan 2013, Toh, Y.C., Blagovic, K., Yu, H. and Voldman, J., 2011. By evaluating NE tissue structural dysmorphia in the micropatterned NE model, we can successfully model gene- and drug-induced neurodevelopmental defects. Dr Toh is a recipient of the Australia Research Council Future Fellowship, National University of Singapore Research Scholarship, A*STAR Graduate Scholarship and A*STAR International Fellowship. 2005,Assay and Drug Development Technologies,3(2): 169-176. Seep Arora, Christine Cheung, Evelyn Yim*, Yi-Chin Toh*, Topography elicits distinct phenotypes and functions in human primary and stem cell derived endothelial cells, 2020, Biomaterials, 234, 119747. This kit was designed with modular components (i.e., each component followed a standardized unit) to achieve (1) customizability (users can easily reconfigure various components to comply with their experiments), (2) forward compatibility (new parts with the standardized unit can be designed and easily interfaced to the current kit), and (3) easy replacement of the parts experiencing wear and tear. By continuing you agree to the use of cookies. Xianzhu Zhang, Dandan Cai, Feifei Zhou, Jie Yu, Xinyu Wu, Dongsheng Yu, Yiwei Zou, Yi Hong, Chunhui Yuan, Yishan Chen, Zongyou Pan, Varitsara Bunpetch, Heng Sun, Chengrui An, Yi-Chin Toh, Hongwei Ouyang, Shufang Zhang, Targeting downstream subcellular YAP activity as a function of matrix stiness with Verteporn-encapsulated chitosan microsphere attenuates osteoarthritis, 2020,Biomaterials, 232: 119724, Terry Ching, Yi-Chin Toh, Michinao Hashimoto, Fabrication of Complex 3D Fluidic Networks via Modularized Stereolithography, 2019, Advanced Engineering Materials, 1901109, https://doi.org/10.1002/adem.201901109.